Research Officer CEMB, University of the Punjab
20 years of experience
I have been serving as a Research Officer at the Centre of Excellence in Molecular Biology (CEMB), Lahore since 2006. I have extensive experience in the field of Molecular Biology. Initially, I worked in the Biopharmaceutical Group at CEMB, focusing on cloning, transformation, purification, and quality control analyses of human recombinant proteins in both prokaryotic and eukaryotic expression systems. Since 2010, he has been a key member of the Seed Biotechnology Laboratory, where my research is dedicated to developing transgenic crops including cotton, potato, sugarcane and soybean with emphasis on resistance to biotic stresses such as pink bollworm and fungal pathogens. I involve in genetic transformation, molecular characterization, and functional validation of transgenes to enhance plant resistance. I am specialize in vector construction, Agrobacterium-mediated transformation and particle bombardment techniques for introducing resistance genes into crops. My research also includes the characterization and screening of the Plant Growth Promoting bacteria
Additionally, I have contributed significantly to the commercialization of genetically modified cotton varieties developed by CEMB. My efforts have played a vital role in developing four double Bt gene and five triple gene (double Bt & GTG) cotton varieties, which provide enhanced protection against insect pests and fungal infections. He is actively engaged in drafting the submission for the National Biosafety Committee, Islamabad, to pursue approval for Genetic Manipulation Lab work, field trials, and the commercialization of genetically modified crops. Additionally, I prepare drafts for the approval of cotton varieties by the Punjab Seed Corporation (PSC) and Sindh Seed Council (SSC). Furthermore, I am also engaged in GMO testing of transgenic cotton varieties referred by both public and private sector organizations. He performs PCR, dipstick, and ELISA tests to evaluate genetically modified cotton varieties, including those in the National Coordinated Varietal Trial (NCVT) conducted by the Pakistan Central Cotton Committee (PCCC).
HPLC, FPLC, Spectrophotometer, SDS PAGE, Agarose Gel Electrophoresis, Gene Gun
https://www.linkedin.com/in/muhammad-tariq-0971ab63/ https://scholar.google.com/citations?user=9pbP9tgAAAAJ&hl=en https://www.cemb.edu.pk/?page_id=14824